Recombinant:Article Title: Orchestration of pluripotent stem cell genome reactivation during mitotic exit.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-H3S10ph Millipore Cat.# 05–1336; RRID:AB_1977261 Donkey anti-mouse IgG-AF647 Invitrogen Cat.# A31571; RRID:AB_162542 Chicken anti-rabbit IgG-AF647 Invitrogen Cat.# A21443; RRID: AB_2535861 Rabbit anti-OCT4 Cell Signaling Technology Cat.# 5677 Rabbit anti-SOX2 Cell Signaling Technology Cat.# 23064; RRID:AB_2714146 Rabbit anti-NANOG Cell Signaling Technology Cat.# 8822; RRID:AB_11217637 Anti H2Av- dmelanogaster Active Motif Cat.#61686; RRID:AB_2737370 Chemicals, peptides, and recombinant proteins CHIR99021 Sigma-Aldrich Cat.# 361559; CAS: 252917-06-9 PD184352 Sigma-Aldrich Cat.# PZ0181; CAS: 212631-79-3 Doxycycline-hydrochloride Sigma-Aldrich Cat.#D3447; CAS: 10592-13-9 Deposited data GSE87822 King et al.41 N/A GSE134680 Friman et al.15 N/A GSE69323 Wamaitha et al.43 N/A GSE241731 with token sfudowksvhmzrud This paper N/A GSE275801 with token idevygmqthubfgl This paper N/A GSE275803 with token wdefyqgsbpmdlgh This paper N/A GSE275804 with token srkpagewfdqjhwp This paper N/A Experimental models: Cell lines CGR8 (ESC Mus musculus) Sigma-Aldrich Cat#07032901; RRID:CVCL_3987 ZHBTc4 (ESC Mus musculus) Niwa et al.36 RRID: CVCL_C715 2TS22C (ESC Mus musculus) Masui et al.37 RRID:CVCL_E266 44iN (ESC Mus musculus) Festuccia et al.38 N/A HEK293T (ESC Mus musculus) ATCC RRID:CVCL_0063 Cdt1-MD (ESC Mus musculus) This study N/A Recombinant DNA pLV-PGK-YPet-MD Deluz et al.3 N/A pLV-EF1a-mCherry-hCdt1(1/100)(Cy-) This study N/A pLV-EF1a-mCherry/PGK-Hygromycin This study N/A psPAX2 Addgene RRID: Addgene_12260 pMD2G Addgene RRID: Addgene_12259 Software and algorithms FiJi Version 2.14.0/1.54f Schindelin et al.49 RRID:SCR_002285 scikit-learn Version 1.4.2 Buitinck et al.50 RRID:SCR_002577 ggplot2 Version 3.5.0 Wickham et al.51 RRID:SCR_014601 STAR Version 2.7.0e Dobin et al.52 RRID:SCR_015899 SAMTools Version 1.10 Li et al.53 RRID:SCR_002105 deepTools Version 3.5.1 Ramı́rez et al.54 RRID:SCR_016366 MACS Version 2.2.4 Zhang et al.55 RRID:SCR_013291 BEDTools Quinlan et al.56 RRID:SCR_012865 Bedops Version 2.4.41 Neph et al.57 RRID:SCR_012865 IGB Freese et al.58 RRID:SCR_011792 (Continued on next page) 16 Cell Reports 44, 115486, April 22, 2025 .. The CGR8 (ECACC 07032901), ZHBTc4,36 2TS22C,37 and 44iN38 mouse embryonic stem cells lines38 were routinely cultured on 0.1% gelatin-coated (Sigma, G9391) 100mm Petri dishes at 37 C and 5% CO2 in GMEM (Sigma, G5154) supplemented with 10% inactivated ESC qualified fetal bovine serum (Gibco, 16141–079), 1% nonessential amino acids (Gibco,11140–050), 2 mM L-glutamine (Gibco, 25030–024), 2 mM sodium pyruvate (Sigma, S8636-100ML), 100mM 2-mercaptoethanol (Sigma, M3148), 1% penicillin and streptomycin (BioConcept, 4–01F00-H), leukemia inhibitory factor (in-house produced and validated for its capacity to maintain pluripotency), 3 mM CHIR99021 (Sigma, 361559) and 0.8 mM PD184352 (Sigma, PZ0181).
Magnetic Cell Separation:Article Title: Orchestration of pluripotent stem cell genome reactivation during mitotic exit.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-H3S10ph Millipore Cat.# 05–1336; RRID:AB_1977261 Donkey anti-mouse IgG-AF647 Invitrogen Cat.# A31571; RRID:AB_162542 Chicken anti-rabbit IgG-AF647 Invitrogen Cat.# A21443; RRID: AB_2535861 Rabbit anti-OCT4 Cell Signaling Technology Cat.# 5677 Rabbit anti-SOX2 Cell Signaling Technology Cat.# 23064; RRID:AB_2714146 Rabbit anti-NANOG Cell Signaling Technology Cat.# 8822; RRID:AB_11217637 Anti H2Av- dmelanogaster Active Motif Cat.#61686; RRID:AB_2737370 Chemicals, peptides, and recombinant proteins CHIR99021 Sigma-Aldrich Cat.# 361559; CAS: 252917-06-9 PD184352 Sigma-Aldrich Cat.# PZ0181; CAS: 212631-79-3 Doxycycline-hydrochloride Sigma-Aldrich Cat.#D3447; CAS: 10592-13-9 Deposited data GSE87822 King et al.41 N/A GSE134680 Friman et al.15 N/A GSE69323 Wamaitha et al.43 N/A GSE241731 with token sfudowksvhmzrud This paper N/A GSE275801 with token idevygmqthubfgl This paper N/A GSE275803 with token wdefyqgsbpmdlgh This paper N/A GSE275804 with token srkpagewfdqjhwp This paper N/A Experimental models: Cell lines CGR8 (ESC Mus musculus) Sigma-Aldrich Cat#07032901; RRID:CVCL_3987 ZHBTc4 (ESC Mus musculus) Niwa et al.36 RRID: CVCL_C715 2TS22C (ESC Mus musculus) Masui et al.37 RRID:CVCL_E266 44iN (ESC Mus musculus) Festuccia et al.38 N/A HEK293T (ESC Mus musculus) ATCC RRID:CVCL_0063 Cdt1-MD (ESC Mus musculus) This study N/A Recombinant DNA pLV-PGK-YPet-MD Deluz et al.3 N/A pLV-EF1a-mCherry-hCdt1(1/100)(Cy-) This study N/A pLV-EF1a-mCherry/PGK-Hygromycin This study N/A psPAX2 Addgene RRID: Addgene_12260 pMD2G Addgene RRID: Addgene_12259 Software and algorithms FiJi Version 2.14.0/1.54f Schindelin et al.49 RRID:SCR_002285 scikit-learn Version 1.4.2 Buitinck et al.50 RRID:SCR_002577 ggplot2 Version 3.5.0 Wickham et al.51 RRID:SCR_014601 STAR Version 2.7.0e Dobin et al.52 RRID:SCR_015899 SAMTools Version 1.10 Li et al.53 RRID:SCR_002105 deepTools Version 3.5.1 Ramı́rez et al.54 RRID:SCR_016366 MACS Version 2.2.4 Zhang et al.55 RRID:SCR_013291 BEDTools Quinlan et al.56 RRID:SCR_012865 Bedops Version 2.4.41 Neph et al.57 RRID:SCR_012865 IGB Freese et al.58 RRID:SCR_011792 (Continued on next page) 16 Cell Reports 44, 115486, April 22, 2025 .. The CGR8 (ECACC 07032901), ZHBTc4,36 2TS22C,37 and 44iN38 mouse embryonic stem cells lines38 were routinely cultured on 0.1% gelatin-coated (Sigma, G9391) 100mm Petri dishes at 37 C and 5% CO2 in GMEM (Sigma, G5154) supplemented with 10% inactivated ESC qualified fetal bovine serum (Gibco, 16141–079), 1% nonessential amino acids (Gibco,11140–050), 2 mM L-glutamine (Gibco, 25030–024), 2 mM sodium pyruvate (Sigma, S8636-100ML), 100mM 2-mercaptoethanol (Sigma, M3148), 1% penicillin and streptomycin (BioConcept, 4–01F00-H), leukemia inhibitory factor (in-house produced and validated for its capacity to maintain pluripotency), 3 mM CHIR99021 (Sigma, 361559) and 0.8 mM PD184352 (Sigma, PZ0181).
|
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 